| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human CTSD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CTSD |
| Gene ID: |
1509 |
| Uniprot ID: |
CATD_HUMAN |
| Specificity: |
This kit recognizes Human CTSD in samples. No significant cross-reactivity or interference between Human CTSD and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level). |
| Post Translational Modifications | N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified. |
| Function | Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. |
| Protein Name | Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-2022377Reactome: R-HSA-2132295Reactome: R-HSA-6798695Reactome: R-HSA-77387Reactome: R-HSA-9018519 |
| Cellular Localisation | LysosomeMelanosomeSecretedExtracellular SpaceIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvIn Aortic SamplesDetected As An Extracellular Protein Loosely Bound To The Matrix |
| Alternative CLIA Names | Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain CLIA kitCTSD CLIA kitCPSD CLIA kit |
| Specificity | This kit recognizes Human CTSD in samples. No significant cross-reactivity or interference between Human CTSD and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-117 | 107 |
| EDTA plasma(n=8) | 100-113 | 107 |
| Cell culture media(n=8) | 97-109 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 47.80 | 135.43 | 338.17 | 49.78 | 136.96 | 323.89 |
| Standard deviation | 4.88 | 12.92 | 36.22 | 6.05 | 13.37 | 31.81 |
| CV (%) | 10.21 | 9.54 | 10.71 | 12.15 | 9.76 | 9.82 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
