| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.1 EU/Mu g |
| Immunoreactivity: | Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl) , in the presence of 5, 5 Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >452.49 pmol/min/Mu g. |
| Gene Symbol: | CFD |
| Gene ID: | 1675 |
| Uniprot ID: | CFAD_HUMAN |
| Immunogen Region: | Ile26-Ala253 |
| Immunogen: | Recombinant Human Complement factor D/CFD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile26-Ala253) of human CFD/Adipsin/PFD (Accession #NP_001919.2.) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | ILGGREAEAHARPYMASVQL NGAHLCGGVLVAEQWVLSAA HCLEDAADGKVQVLLGAHSL SQPEPSKRLYDVLRAVPHPD SQPDTIDHDLLLLQLSEKAT LGPAVRPLPWQRVDRDVAPG TLCDVAGWGIVNHAGRRPDS LQHVLLPVLDRATCNRRTHH DGAITERLMCAESNRRDSCK GDSGGPLVCGGVLEGVVTSG SRVCGNRKKPGIYTRVASYA AWIDSVLA |
| Function | Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3. The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases. Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway. |
| Protein Name | Complement Factor DAdipsinC3 Convertase ActivatorProperdin Factor D |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-173736Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Complement Factor D proteinAdipsin proteinC3 Convertase Activator proteinProperdin Factor D proteinCFD proteinDF proteinPFD protein |
Information sourced from Uniprot.org
