| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: |
CLEC12A |
| Gene ID: |
160364 |
| Uniprot ID: |
CL12A_HUMAN |
| Immunogen: |
Recombinant Human CLL-1/MICL/CD371 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His75-Ala275) of human CLEC12A (Accession #NP_001193939.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: |
HVTLKIEMKKMNKLQNISEE LQRNISLQLMSNMNISNKIR NLSTTLQTIATKLCRELYSK EQEHKCKPCPRRWIWHKDSC YFLSDDVQTWQESKMACAAQ NASLLKINNKNALEFIKSQS RSYDYWLGLSPEEDSTRGMR VDNIINSSAWVIRNAPDLNN MYCGYINRLYVQYYHCTYKK RMICEKMANPVQLGSTYFRE A |
| Tissue Specificity | Preferentially expressed in lymphoid tissues and immune cells, including natural killer (NK) cells, T-cells, dendritic cells and monocytes or macrophages. Detected in spleen macrophage-rich red pulp and in lymph node (at protein level). Detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes and macrophages. |
| Post Translational Modifications | Phosphorylated at Tyr-7 by SRC in the ITIM motif following ligand-binding, promoting recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11. Highly N-glycosylated.glycosylation varies between cell types. |
| Function | Myeloid inhibitory C-type lectin receptor that acts as a negative regulator of myeloid cell activation. Myeloid cell inhibition is required to limit proinflammatory pathways and protect against excessive inflammation. Specifically recognizes and binds various structures, such as neutrophil extracellular traps (NETs) or monosodium urate crystals. Also acts as a pattern-recognition receptor for pathogen-associated molecules, such as plasmodium hemozoin or mycobacterial micolic acid. Ligand-binding induces phosphorylation of its ITIM motif, followed by recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11, which counteract tyrosine-protein kinase SYK, thereby preventing myeloid cell activation. Acts as a pattern-recognition receptor for NETs in neutrophils: specifically recognizes DNA in NETs, leading to inhibit neutrophil activation and limit further NET formation. This regulation is essential for controlling key neutrophil responses and limit NET-mediated inflammatory conditions. Also recognizes dead cells by acting as a receptor for monosodium urate crystals, leading to down-regulate neutrophil activation. Binding to monosodium urate crystals also promotes the type I interferon response. Acts as an inhibitor of natural killer (NK) cell cytotoxicity. Also acts as an ihibitor of dendritic cell maturation in an IL10-dependent manner. |
| Protein Name | C-Type Lectin Domain Family 12 Member AC-Type Lectin-Like Molecule 1Cll-1Dendritic Cell-Associated Lectin 2Dcal-2Killer Cell C-Type Lectin-Like Receptor L1Hklrl1Myeloid Inhibitory C-Type Lectin-Like ReceptorMiclCd Antigen Cd371 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinLigand Binding Leads To InternalizationClusters At Phagocytic Vesicles Upon Monosodium Urate Crystal-Binding |
| Alternative Protein Names | C-Type Lectin Domain Family 12 Member A proteinC-Type Lectin-Like Molecule 1 proteinCll-1 proteinDendritic Cell-Associated Lectin 2 proteinDcal-2 proteinKiller Cell C-Type Lectin-Like Receptor L1 proteinHklrl1 proteinMyeloid Inhibitory C-Type Lectin-Like Receptor proteinMicl proteinCd Antigen Cd371 proteinCLEC12A proteinCLL1 proteinDCAL2 proteinKLRL1 proteinMICL protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
