| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to inhibit Anti-CD3-induced proliferation of jurkat. The ED50 for this effect is 24-96 ng/mL. |
| Gene Symbol: | BTN3A1 |
| Gene ID: | 11119 |
| Uniprot ID: | BT3A1_HUMAN |
| Immunogen Region: | Gln30-Gly254 |
| Immunogen: | Recombinant human BTN3A1/CD277 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln30-Gly254) of human BTN3A1/CD277 (Accession #NP_919423.1) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Sequence: | QFSVLGPSGPILAMVGEDAD LPCHLFPTMSAETMELKWVS SSLRQVVNVYADGKEVEDRQ SAPYRGRTSILRDGITAGKA ALRIHNVTASDSGKYLCYFQ DGDFYEKALVELKVAALGSD LHVDVKGYKDGGIHLECRST GWYPQPQIQWSNNKGENIPT VEAPVVADGVGLYAVAASVI MRGSSGEGVSCTIRSSLLGL EKTASISIADPFFRSAQRWI AALAG |
| Post Translational Modifications | N-glycosylated. |
| Function | Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate. |
| Protein Name | Butyrophilin Subfamily 3 Member A1Cd Antigen Cd277 |
| Database Links | Reactome: R-HSA-8851680 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Butyrophilin Subfamily 3 Member A1 proteinCd Antigen Cd277 proteinBTN3A1 proteinBTF5 protein |
Information sourced from Uniprot.org