| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This BMPR2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
BMPR2 |
| Gene ID: |
659 |
| Uniprot ID: |
BMPR2_HUMAN |
| Specificity: |
This kit recognizes Human BMPR2 in samples. No significant cross-reactivity or interference between Human BMPR2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Can also mediate signaling through the activation of the p38MAPK cascade. Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6. |
| Protein Name | Bone Morphogenetic Protein Receptor Type-2Bmp Type-2 ReceptorBmpr-2Bone Morphogenetic Protein Receptor Type IiBmp Type Ii ReceptorBmpr-Ii |
| Database Links | Reactome: R-HSA-201451 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Bone Morphogenetic Protein Receptor Type-2 ELISA kitBmp Type-2 Receptor ELISA kitBmpr-2 ELISA kitBone Morphogenetic Protein Receptor Type Ii ELISA kitBmp Type Ii Receptor ELISA kitBmpr-Ii ELISA kitBMPR2 ELISA kitPPH1 ELISA kit |
| Specificity | This kit recognizes Human BMPR2 in samples. No significant cross-reactivity or interference between Human BMPR2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-96 | 90 |
| EDTA plasma (n=8) | 88-103 | 95 |
| Cell culture media (n=8) | 87-101 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.53 | 1.10 | 3.37 | 0.56 | 1.11 | 3.14 |
| Standard deviation | 0.04 | 0.05 | 0.12 | 0.03 | 0.05 | 0.14 |
| CV (%) | 7.55 | 4.55 | 3.56 | 5.36 | 4.50 | 4.46 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
