Human BCKDHA protein (Recombinant) (His-Tag) (STJP017081)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human BCKDHA-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% Glycerol, 0.2M NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCKDHA |
| Gene ID | 593 |
| Uniprot ID | ODBA_HUMAN |
| Accession Number | NP_000700.1 |
| Immunogen | Human |
| Immunogen Region | 46-445aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-337 by BCKDK and dephosphorylated by protein phosphatase PPM1K. |
| Function | Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction. |
| Protein Name | 2-Oxoisovalerate Dehydrogenase Subunit Alpha - MitochondrialBranched-Chain Alpha-Keto Acid Dehydrogenase E1 Component Alpha ChainBckde1aBckdh E1-Alpha |
| Database Links | Reactome: R-HSA-70895Reactome: R-HSA-9859138Reactome: R-HSA-9865113Reactome: R-HSA-9865125Reactome: R-HSA-9907570Reactome: R-HSA-9912481Reactome: R-HSA-9912529 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Protein Names | 2-Oxoisovalerate Dehydrogenase Subunit Alpha - Mitochondrial proteinBranched-Chain Alpha-Keto Acid Dehydrogenase E1 Component Alpha Chain proteinBckde1a proteinBckdh E1-Alpha proteinBCKDHA protein |
Information sourced from Uniprot.org