• Human BACE-1/ASP2 protein (Recombinant) (C-His) (STJP000940)

Human BACE-1/ASP2 protein (Recombinant) (C-His) (STJP000940)

SKU:
STJP000940

£80.50 - £257.50
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Host: HEK293 cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: < 0.1 EU/Mu g of the protein by LAL method.
Immunoreactivity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK (Dpn) RR-NH2. The specific activity is >5.5 pmol/min/Mu g.
Gene Symbol: BACE1
Gene ID: 23621
Uniprot ID: BACE1_HUMAN
Immunogen: Recombinant Human BACE-1/ASP2 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Thr22-Tyr460) of human BACE1/ASP2 (Accession #NP_036236.1) fused with an 8×His tag at the C-terminus.
Immunogen Sequence: TQHGIRLPLRSGLGGAPLGL RLPRETDEEPEEPGRRGSFV EMVDNLRGKSGQGYYVEMTV GSPPQTLNILVDTGSSNFAV GAAPHPFLHRYYQRQLSSTY RDLRKGVYVPYTQGKWEGEL GTDLVSIPHGPNVTVRANIA AITESDKFFINGSNWEGILG LAYAEIARPDDSLEPFFDSL VKQTHVPNLFSLQLCGAGFP LNQSEVLASVGGSMIIGGID HSLYTGSLWYTPIRREWYY
Tissue Specificity Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Post Translational Modifications N-Glycosylated. Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Palmitoylation mediates lipid raft localization. Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation. Monoubiquitinated and 'Lys-63'-linked polyubitinated. Deubiquitnated by USP8.inhibits lysosomal degradation. Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface.
Function Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1.
Protein Name Beta-Secretase 1
Aspartyl Protease 2
Asp2
Asp 2
Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1
Beta-Site App Cleaving Enzyme 1
Memapsin-2
Membrane-Associated Aspartic Protease 2
Database Links Reactome: R-HSA-977225
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus
Trans-Golgi Network
Endoplasmic Reticulum
Endosome
Cell Surface
Cytoplasmic Vesicle Membrane
Membrane Raft
Lysosome
Late Endosome
Early Endosome
Recycling Endosome
Cell Projection
Axon
Dendrite
Predominantly Localized To The Later Golgi/Trans-Golgi Network (Tgn) And Minimally Detectable In The Early Golgi Compartments
A Small Portion Is Also Found In The Endoplasmic Reticulum
Endosomes And On The Cell Surface
Colocalization With App In Early Endosomes Is Due To Addition Of Bisecting N-Acetylglucosamine Wich Blocks Targeting To Late Endosomes And Lysosomes
Retrogradly Transported From Endosomal Compartments To The Trans-Golgi Network In A Phosphorylation- And Gga1- Dependent Manner
Alternative Protein Names Beta-Secretase 1 protein
Aspartyl Protease 2 protein
Asp2 protein
Asp 2 protein
Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 protein
Beta-Site App Cleaving Enzyme 1 protein
Memapsin-2 protein
Membrane-Associated Aspartic Protease 2 protein
BACE1 protein
BACE protein
KIAA1149 protein

Information sourced from Uniprot.org

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