• Human Autotaxin/E-NPP2 protein (Recombinant) (C-His) (STJP002054)

Human Autotaxin/E-NPP2 protein (Recombinant) (C-His) (STJP002054)

SKU:
STJP002054

£106.50 - £359.50
Current Stock:
Host: HEK293 cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from a 0.2 Mu m filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: < 1 EU/Mu g of the protein by LAL method.
Immunoreactivity: Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >15000 pmol/min/Mu g, as measured under the described conditions.
Gene Symbol: ENPP2
Gene ID: 5168
Uniprot ID: ENPP2_HUMAN
Immunogen Region: Ala36-Ile863
Immunogen: Recombinant Human Autotaxin/E-NPP2 Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Ala36-Ile863) of human ENPP-2 (Accession #AAH34961.1) fused with a 6xHis tag at the C-terminus.
Immunogen Sequence: DSPWTNISGSCKGRCFELQE AGPPDCRCDNLCKSYTSCCH DFDELCLKTARGWECTKDRC GEVRNEENACHCSEDCLARG DCCTNYQVVCKGESHWVDDD CEEIKAAECPAGFVRPPLII FSVDGFRASYMKKGSKVMPN IEKLRSCGTHSPYMRPVYPT KTFPNLYTLATGLYPESHGI VGNSMYDPVFDATFHLRGRE KFNHRWWGGQPLWITATKQG VKAGTFFWSVVIPHERRIL
Post Translational Modifications N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. The interdomain disulfide bond between Cys-414 and Cys-806 is essential for catalytic activity.
Function Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Its major substrate is lysophosphatidylcholine. Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF).
Protein Name Autotaxin
Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2
E-Npp 2
Extracellular Lysophospholipase D
Lysopld
Database Links Reactome: R-HSA-199220
Cellular Localisation Secreted
Alternative Protein Names Autotaxin protein
Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 protein
E-Npp 2 protein
Extracellular Lysophospholipase D protein
Lysopld protein
ENPP2 protein
ATX protein
PDNP2 protein

Information sourced from Uniprot.org

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