| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human ANXA5 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ANXA5 |
| Gene ID: |
308 |
| Uniprot ID: |
ANXA5_HUMAN |
| Specificity: |
This kit recognizes Human ANXA5 in samples. No significant cross-reactivity or interference between Human ANXA5 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Function | This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. |
| Protein Name | Annexin A5Anchorin CiiAnnexin VAnnexin-5Calphobindin ICpb-IEndonexin IiLipocortin VPlacental Anticoagulant Protein 4Pp4Placental Anticoagulant Protein IPap-IThromboplastin InhibitorVascular Anticoagulant-AlphaVac-Alpha |
| Database Links | Reactome: R-HSA-114608 |
| Alternative CLIA Names | Annexin A5 CLIA kitAnchorin Cii CLIA kitAnnexin V CLIA kitAnnexin-5 CLIA kitCalphobindin I CLIA kitCpb-I CLIA kitEndonexin Ii CLIA kitLipocortin V CLIA kitPlacental Anticoagulant Protein 4 CLIA kitPp4 CLIA kitPlacental Anticoagulant Protein I CLIA kitPap-I CLIA kitThromboplastin Inhibitor CLIA kitVascular Anticoagulant-Alpha CLIA kitVac-Alpha CLIA kitANXA5 CLIA kitANX5 CLIA kitENX2 CLIA kitPP4 CLIA kit |
| Specificity | This kit recognizes Human ANXA5 in samples. No significant cross-reactivity or interference between Human ANXA5 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-106 | 99 |
| EDTA plasma(n=8) | 86-100 | 92 |
| Cell culture media(n=8) | 94-107 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 198.97 | 362.03 | 1960.13 | 204.27 | 395.76 | 1829.49 |
| Standard deviation | 24.21 | 33.42 | 214.63 | 23.92 | 32.21 | 140.14 |
| CV (%) | 12.17 | 9.23 | 10.95 | 11.71 | 8.14 | 7.66 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
