Human Aminopeptidase N/CD13 protein (Recombinant) (C-His) (STJP002597)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Aminopeptidase N/CD13 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys69-Lys967) of human ANPEP/CD13 (Accession #NP_001141.2) fused with an 8ΓHis tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Aminopeptidase N/CD13-C-His protein was developed in hek293 cells using the region Lys69-Lys967. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >4300 pmol/min/Mu g. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | ANPEP |
| Gene ID | 290 |
| Uniprot ID | AMPN_HUMAN |
| Immunogen | Recombinant Human Aminopeptidase N/CD13 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys69-Lys967) of human ANPEP/CD13 (Accession #NP_001141.2) fused with an 8ΓHis tag at the C-terminus. |
| Immunogen Region | Lys69-Lys967 |
| Immunogen Sequence | KAWNRYRLPNTLKPDSYRVT LRPYLTPNDRGLYVFKGSST VRFTCKEATDVIIIHSKKLN YTLSQGHRVVLRGVGGSQPP DIDKTELVEPTEYLVVHLKG SLVKDSQYEMDSEFEGELAD DLAGFYRSEYMEGNVRKVVA TTQMQAADARKSFPCFDEPA MKAEFNITLIHPKDLTALSN MLPKGPSTPLPEDPNWNVTE FHTTPKMSTYLLAFIVSEFD YVEKQASNGVLIRIWARPS |
Additional Info
| Post Translational Modifications | Sulfated. N- and O-glycosylated. May undergo proteolysis and give rise to a soluble form. |
| Function | Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity. (Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. (Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection. |
| Protein Name | Aminopeptidase NAp-NHapnAlanyl AminopeptidaseAminopeptidase MAp-MMicrosomal AminopeptidaseMyeloid Plasma Membrane Glycoprotein Cd13Gp150Cd Antigen Cd13 |
| Database Links | Reactome: R-HSA-2022377Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinAlso Found As A Soluble Form |
| Alternative Protein Names | Aminopeptidase N proteinAp-N proteinHapn proteinAlanyl Aminopeptidase proteinAminopeptidase M proteinAp-M proteinMicrosomal Aminopeptidase proteinMyeloid Plasma Membrane Glycoprotein Cd13 proteinGp150 proteinCd Antigen Cd13 proteinANPEP proteinAPN proteinCD13 proteinPEPN protein |
Information sourced from Uniprot.org