Human Aldo-keto Reductase 1C4/AKR1C4 protein (Recombinant) (His-Tag) (STJP017241)

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STJP017241
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Host: E.coli
Applications: SDS-PAGE/Enzyme Activity
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Recombinant-Human Aldo-keto Reductase 1C4/AKR1C4-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
Formulation: Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 20% Glycerol, 0.1M NaCl, 1mM DTT
Concentration: 1 mg/mL
Storage Instruction: For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Immunoreactivity: Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
Gene Symbol: AKR1C4
Gene ID: 1109
Uniprot ID: AK1C4_HUMAN
Immunogen Region: 1-323aa
Accession Number: NP_001809
Immunogen: Human
Immunogen Sequence: MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKS
Tissue Specificity Liver specific.
Post Translational Modifications The N-terminus is blocked.
Function Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Liver specific enzyme that acts as an NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain. Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH. Acts preferentially as a 3-alpha-hydroxysteroid dehydrogenase (HSD) with a subsidiary 3-beta-HSD activity. Catalyzes efficiently the transformation of the potent androgen 5-alpha-dihydrotestosterone (5alpha-DHT or 17beta-hydroxy-5alpha-androstan-3-one) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Catalyzes the reduction of estrone into 17beta-estradiol but with low efficiency. Metabolizes a broad spectrum of natural and synthetic therapeutic steroid and plays an important role in metabolism of androgens, estrogens, progestereone and conjugated steroids. Catalyzes the biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leading to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.
Protein Name Aldo-Keto Reductase Family 1 Member C4
3-Alpha-Hydroxysteroid Dehydrogenase Type I
3-Alpha-Hsd1
3alpha-Hydroxysteroid 3-Dehydrogenase
Chlordecone Reductase
Cdr
Dihydrodiol Dehydrogenase 4
Dd-4
Dd4
Hakra
Database Links Reactome: R-HSA-193368
Reactome: R-HSA-193775
Reactome: R-HSA-193807
Reactome: R-HSA-975634
Cellular Localisation Cytoplasm
Cytosol
Alternative Protein Names Aldo-Keto Reductase Family 1 Member C4 protein
3-Alpha-Hydroxysteroid Dehydrogenase Type I protein
3-Alpha-Hsd1 protein
3alpha-Hydroxysteroid 3-Dehydrogenase protein
Chlordecone Reductase protein
Cdr protein
Dihydrodiol Dehydrogenase 4 protein
Dd-4 protein
Dd4 protein
Hakra protein
AKR1C4 protein
CHDR protein

Information sourced from Uniprot.org

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