| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This human ADIPOR2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ADIPOR2 |
| Gene ID: |
79602 |
| Uniprot ID: |
PAQR2_HUMAN |
| Specificity: |
This kit recognizes Human ADIPOR2 in samples. No significant cross-reactivity or interference between Human ADIPOR2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Ubiquitous. Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung. |
| Function | Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal body fat and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels. |
| Protein Name | Adiponectin Receptor Protein 2Progestin And Adipoq Receptor Family Member 2Progestin And Adipoq Receptor Family Member Ii |
| Database Links | Reactome: R-HSA-163680 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLocalized To The Cell Membrane And Intracellular Organelles |
| Alternative CLIA Names | Adiponectin Receptor Protein 2 CLIA kitProgestin And Adipoq Receptor Family Member 2 CLIA kitProgestin And Adipoq Receptor Family Member Ii CLIA kitADIPOR2 CLIA kitPAQR2 CLIA kit |
| Specificity | This kit recognizes Human ADIPOR2 in samples. No significant cross-reactivity or interference between Human ADIPOR2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-111 | 101 |
| EDTA plasma(n=8) | 96-112 | 104 |
| Cell culture media(n=8) | 91-102 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.55 | 1.15 | 4.89 | 0.54 | 1.22 | 4.86 |
| Standard deviation | 0.04 | 0.10 | 0.52 | 0.05 | 0.10 | 0.40 |
| CV (%) | 7.27 | 8.70 | 10.63 | 9.26 | 8.20 | 8.23 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
