| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human ACO1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ACO1 |
| Gene ID: |
48 |
| Uniprot ID: |
ACOHC_HUMAN |
| Specificity: |
This kit recognizes Human ACO1 in samples. No significant cross-reactivity or interference between Human ACO1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA. Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate. |
| Protein Name | Cytoplasmic Aconitate HydrataseAconitaseCitrate Hydro-LyaseFerritin Repressor ProteinIron Regulatory Protein 1Irp1Iron-Responsive Element-Binding Protein 1Ire-Bp 1 |
| Database Links | Reactome: R-HSA-917937 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative CLIA Names | Cytoplasmic Aconitate Hydratase CLIA kitAconitase CLIA kitCitrate Hydro-Lyase CLIA kitFerritin Repressor Protein CLIA kitIron Regulatory Protein 1 CLIA kitIrp1 CLIA kitIron-Responsive Element-Binding Protein 1 CLIA kitIre-Bp 1 CLIA kitACO1 CLIA kitIREB1 CLIA kit |
| Specificity | This kit recognizes Human ACO1 in samples. No significant cross-reactivity or interference between Human ACO1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-106 | 100 |
| EDTA plasma(n=8) | 98-110 | 104 |
| Cell culture media(n=8) | 92-105 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 106.87 | 286.48 | 886.35 | 115.85 | 276.99 | 826.38 |
| Standard deviation | 11.42 | 28.39 | 70.64 | 12.91 | 25.46 | 50.24 |
| CV (%) | 10.69 | 9.91 | 7.97 | 11.14 | 9.19 | 6.08 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
