| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | CXCR2 Positive Control for STJ500651 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: | WB: 1:500 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | CCR2 |
| Gene ID: | 729230 |
| Uniprot ID: | CCR2_HUMAN |
| Specificity: | This is positive control is recommended for use in combination with CXCR2 antibody STJ500651. |
| Tissue Specificity | Expressed by monocytes and IL2-activated NK cells. Abundantly expressed on CD14+/CD16- monocytes and weakly on CD14+/CD16+ monocytes, type 2 dendritic cells (DCs) and plasmacytoid DCs (at protein level). |
| Post Translational Modifications | N-glycosylated. Sulfation increases the affinity for both monomeric and dimeric CCL2 with stronger binding to the monomeric form. Binding of sulfated CCR2 to CCL2 promotes conversion of CCL2 from dimer to monomer. |
| Function | Key functional receptor for CCL2 but can also bind CCL7, and CCL12. Also transduces signaling mediated by CCL13. Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion. Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B. Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation. Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression.signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B. Mediates the recruitment of macrophages and monocytes to the injury site following brain injury. (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection. |
| Peptide Name | C-C Chemokine Receptor Type 2 C-C Ckr-2 Cc-Ckr-2 Ccr-2 Ccr2 Monocyte Chemoattractant Protein 1 Receptor Mcp-1-R Cd Antigen Cd192 |
| Database Links | Reactome: R-HSA-1461957 Reactome: R-HSA-380108 Reactome: R-HSA-418594 Reactome: R-HSA-6783783 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein The Chemoattractant Receptors Are Distributed Throughout The Cell Surface After Stimulation With A Ligand Such As Ccl2 They Are Rapidly Recruited Into Microdomain Clusters At The Cell Membrane |
| Alternative Peptide Names | C-C Chemokine Receptor Type 2 protein C-C Ckr-2 protein Cc-Ckr-2 protein Ccr-2 protein Ccr2 protein Monocyte Chemoattractant Protein 1 Receptor protein Mcp-1-R protein Cd Antigen Cd192 protein CCR2 protein CMKBR2 protein |
Information sourced from Uniprot.org