| Host: |
Rabbit |
| Applications: |
ELISA/IHC/IP/WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ZIP Kinase (400-448) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Purification: |
Affinity Purified |
| Concentration: |
0.5-1.5 µg/µl |
| Dilution Range: |
WB: 1:2, 500-1:5, 000ELISA: 1:50, 000IP: 1:200IHC: 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Dapk3 |
| Gene ID: |
13144 |
| Uniprot ID: |
DAPK3_MOUSE |
| Immunogen Region: |
400-448 |
| Immunogen: |
Synthetic peptide taken within amino acid region on 400-448 on mouse Death-associated protein kinase 3 protein. |
| Tissue Specificity | Highly expressed in heart, brain, lung, skeletal muscle, kidney and testis. Lower levels in liver and spleen. |
| Post Translational Modifications | Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus. The phosphorylation status is critical for kinase activity, oligomerization and intracellular localization. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. The phosphorylated form is localized in the cytoplasm and nuclear translocation or retention is maximal when it is not phosphorylated. Phosphorylation increases the trimeric form, and its dephosphorylation favors a kinase-inactive monomeric form. |
| Function | Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in formation of promyelocytic leukemia protein nuclear body (PML-NB). Involved in apoptosis involving PAWR which mediates cytoplasmic relocation.in vitro phosphorylates PAWR. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton such as in regulation of cell polarity and cell migration. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2.disrupts the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates STAT3 and enhances its transcriptional activity. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. |
| Protein Name | Death-Associated Protein Kinase 3Dap Kinase 3Dap-Like KinaseDlkMypt1 KinaseZip-Kinase |
| Cellular Localisation | NucleusPml BodyCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeChromosomeCentromerePredominantly Localized To The NucleusRelocates To The Cytoplasm On Binding Pawr Where The Complex Appears To Interact With Actin FilamentsAssociated With The Centrosomes Throughout The Mitotic Cell CycleWith The Centromeres From Prophase To Anaphase And With The Contractile Ring During Cytokinesis |
| Alternative Antibody Names | Anti-Death-Associated Protein Kinase 3 antibodyAnti-Dap Kinase 3 antibodyAnti-Dap-Like Kinase antibodyAnti-Dlk antibodyAnti-Mypt1 Kinase antibodyAnti-Zip-Kinase antibodyAnti-Dapk3 antibodyAnti-Zipk antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
