ZIP Kinase Blocking Peptide for STJ500732 is synthetically produced from the 400-448 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino acid region on 400-448 on mouse Death-associated protein kinase 3 protein.
Immunogen Region
400-448
Specificity
This blocking peptide is recommended for use in combination with ZIP Kinase antibody, STJ500732
Additional Info
Tissue Specificity
Highly expressed in heart, brain, lung, skeletal muscle, kidney and testis. Lower levels in liver and spleen.
Post Translational Modifications
Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus. The phosphorylation status is critical for kinase activity, oligomerization and intracellular localization. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. The phosphorylated form is localized in the cytoplasm and nuclear translocation or retention is maximal when it is not phosphorylated. Phosphorylation increases the trimeric form, and its dephosphorylation favors a kinase-inactive monomeric form.
Function
Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in formation of promyelocytic leukemia protein nuclear body (PML-NB). Involved in apoptosis involving PAWR which mediates cytoplasmic relocation.in vitro phosphorylates PAWR. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton such as in regulation of cell polarity and cell migration. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2.disrupts the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates STAT3 and enhances its transcriptional activity. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition.
Peptide Name
Death-Associated Protein Kinase 3Dap Kinase 3Dap-Like KinaseDlkMypt1 KinaseZip-Kinase
Cellular Localisation
NucleusPml BodyCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeChromosomeCentromerePredominantly Localized To The NucleusRelocates To The Cytoplasm On Binding Pawr Where The Complex Appears To Interact With Actin FilamentsAssociated With The Centrosomes Throughout The Mitotic Cell CycleWith The Centromeres From Prophase To Anaphase And With The Contractile Ring During Cytokinesis
Alternative Peptide Names
Death-Associated Protein Kinase 3 proteinDap Kinase 3 proteinDap-Like Kinase proteinDlk proteinMypt1 Kinase proteinZip-Kinase proteinDapk3 proteinZipk protein
