• Immunohistochemistry analysis of paraffin-embedded mouse brain using ZEB2 Rabbit polyclonal antibody (STJ29919) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using ZEB2 Rabbit polyclonal antibody (STJ29919) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ZEB2 antibody (STJ29919) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ZEB2 antibody (1050-1214) (STJ29919)

SKU:
STJ29919

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZEB2 (1050-1214) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZEB2
Gene ID: 9839
Uniprot ID: ZEB2_HUMAN
Immunogen Region: 1050-1214
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1050-1214 of human ZEB2 (NP_055610.1).
Immunogen Sequence: SGEKPYQCDKCGKRFSHSGS YSQHMNHRYSYCKREAEERE AAEREAREKGHLEPTELLMN RAYLQSITPQGYSDSEERES MPRDGESEKEHEKEGEDGYG KLGRQDGDEEFEEEEEESEN KSMDTDPETIRDEEETGDHS MDDSSEDGKMETKSDHEEDN MEDGM
Post Translational Modifications Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.
Function Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin. Represses expression of MEOX2.
Protein Name Zinc Finger E-Box-Binding Homeobox 2
Smad-Interacting Protein 1
Smadip1
Zinc Finger Homeobox Protein 1b
Database Links Reactome: R-HSA-9762293
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Zinc Finger E-Box-Binding Homeobox 2 antibody
Anti-Smad-Interacting Protein 1 antibody
Anti-Smadip1 antibody
Anti-Zinc Finger Homeobox Protein 1b antibody
Anti-ZEB2 antibody
Anti-KIAA0569 antibody
Anti-SIP1 antibody
Anti-ZFHX1B antibody
Anti-ZFX1B antibody
Anti-HRIHFB2411 antibody

Information sourced from Uniprot.org

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