| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ZC3HC1 (1-260) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ZC3HC1 |
| Gene ID: |
51530 |
| Uniprot ID: |
ZC3C1_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZC3HC1 (NP_057562.3). |
| Immunogen Sequence: |
MAAPCEGQAFAVGVEKNWGA VVRSPEGTPQKIRQLIDEGI APEEGGVDAKDTSATSQSVN GSPQAEQPSLESTSKEAFFS RVETFSSLKWAGKPFELSPL VCAKYGWVTVECDMLKCSSC QAFLCASLQPAFDFDRYKQR CAELKKALCTAHEKFCFWPD SPSPDRFGMLPLDEPAILVS EFLDRFQSLCHLDLQLPSLR PEDLKTMCLTEDKISLLLHL LEDELDHRTDERKTTIKLG |
| Tissue Specificity | Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes. |
| Post Translational Modifications | Phosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-354 phosphorylation, a major site during the course of cell-cycle-dependent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry. |
| Function | Essential component of a SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. |
| Protein Name | Zinc Finger C3hc-Type Protein 1Nuclear-Interacting Partner Of AlkHnipaNuclear-Interacting Partner Of Anaplastic Lymphoma Kinase |
| Database Links | Reactome: R-HSA-9725371 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Zinc Finger C3hc-Type Protein 1 antibodyAnti-Nuclear-Interacting Partner Of Alk antibodyAnti-Hnipa antibodyAnti-Nuclear-Interacting Partner Of Anaplastic Lymphoma Kinase antibodyAnti-ZC3HC1 antibodyAnti-NIPA antibodyAnti-HSPC216 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")