| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-ZBTB7A/FBI-1/LRF (450-550) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S3MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:3000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ZBTB7A |
| Gene ID: |
51341 |
| Uniprot ID: |
ZBT7A_HUMAN |
| Immunogen Region: |
450-550 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 450-550 of human ZBTB7A/FBI-1/LRF (O95365). |
| Immunogen Sequence: |
NYDLKNHMRVHTGLRPYQCD SCCKTFVRSDHLHRHLKKDG CNGVPSRRGRKPRVRGGAPD PSPGATATPGAPAQPSSPDA RRNGQEKHFKDEDEDEDVAS P |
| Tissue Specificity | Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level). Expressed in adipose tissues. |
| Post Translational Modifications | Sumoylated. Undergoes sumoylation with SUMO1 that may regulate its transcriptional activity. |
| Function | Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation. Directly and specifically binds to the consensus sequence 5'-GACAGACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions. Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms. That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300. Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors. Also directly interacts with transcription factors like SP1 to prevent their binding to DNA. Functions as an androgen receptor/AR transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements/ARE on target genes. Thereby, negatively regulates androgen receptor signaling and androgen-induced cell proliferation. Involved in the switch between fetal and adult globin expression during erythroid cells maturation. Through its interaction with the NuRD complex regulates chromatin at the fetal globin genes to repress their transcription. Specifically represses the transcription of the tumor suppressor ARF isoform from the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation. Regulates chondrogenesis through the transcriptional repression of specific genes via a mechanism that also requires histone deacetylation. Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis. Involved in adipogenesis through the regulation of genes involved in adipocyte differentiation. Plays a key role in the differentiation of lymphoid progenitors into B and T lineages. Promotes differentiation towards the B lineage by inhibiting the T-cell instructive Notch signaling pathway through the specific transcriptional repression of Notch downstream target genes. Also regulates osteoclast differentiation. May also play a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining/cNHEJ. Recruited to double-strand break sites on damage DNA, interacts with the DNA-dependent protein kinase complex and directly regulates its stability and activity in DNA repair. May also modulate the splicing activity of KHDRBS1 toward BCL2L1 in a mechanism which is histone deacetylase-dependent and thereby negatively regulates the pro-apoptotic effect of KHDRBS1. |
| Protein Name | Zinc Finger And Btb Domain-Containing Protein 7aFactor Binding Ist Protein 1Fbi-1Factor That Binds To Inducer Of Short Transcripts Protein 1Hiv-1 1st-Binding Protein 1Leukemia/Lymphoma-Related FactorPoz And Krueppel Erythroid Myeloid Ontogenic FactorPok Erythroid Myeloid Ontogenic FactorPokemonPokemon 1Ttf-I-Interacting Peptide 21Tip21Zinc Finger Protein 857a |
| Cellular Localisation | NucleusRecruited To Double-Strand Break Sites Of Damaged Dna |
| Alternative Antibody Names | Anti-Zinc Finger And Btb Domain-Containing Protein 7a antibodyAnti-Factor Binding Ist Protein 1 antibodyAnti-Fbi-1 antibodyAnti-Factor That Binds To Inducer Of Short Transcripts Protein 1 antibodyAnti-Hiv-1 1st-Binding Protein 1 antibodyAnti-Leukemia/Lymphoma-Related Factor antibodyAnti-Poz And Krueppel Erythroid Myeloid Ontogenic Factor antibodyAnti-Pok Erythroid Myeloid Ontogenic Factor antibodyAnti-Pokemon antibodyAnti-Pokemon 1 antibodyAnti-Ttf-I-Interacting Peptide 21 antibodyAnti-Tip21 antibodyAnti-Zinc Finger Protein 857a antibodyAnti-ZBTB7A antibodyAnti-FBI1 antibodyAnti-LRF antibodyAnti-ZBTB7 antibodyAnti-ZNF857A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
