• Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody (STJ11101704) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g YY1 antibody (STJ11101704). Western blot was performed from the immunoprecipitate using YY1 antibody (STJ11101704) at a dilution of 1:2000.
  • Confocal imaging of HeLa cells using YY1 Rabbit monoclonal antibody (STJ11101704, dilution 1:50) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Western blot analysis of various lysates using YY1 Rabbit monoclonal antibody (STJ11101704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using YY1 Rabbit monoclonal antibody (STJ11101704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-YY1 antibody (200-300) [S4MR] (STJ11101704)

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STJ11101704

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-YY1 (200-300) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:1000-1:5000
ChIP 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YY1
Gene ID: 7528
Uniprot ID: TYY1_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human YY1 (P25490).
Immunogen Sequence: PGNKKWEQKQVQIKTLEGEF SVTMWSSDEKKDIDHETVVE EQIIGENSPPDYSEYMTGKK LPPGGIPGIDLSDPKQLAEF ARMKPRKIKEDDAPRTIACP H
Post Translational Modifications Phosphorylation at Ser-118 by CK2 prevents proteolytic cleavage by caspase-7 (CASP7) during apoptosis. Proteolytically cleaved by caspase-7 (CASP7) in response to apoptosis. Phosphorylation at Ser-118 protects against proteolytic cleavage. Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites. Ubiquitinated.
Function Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'.some genes have been shown to contain a longer binding motif allowing enhanced binding.the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.proposed to target the INO80 complex to YY1-responsive elements.
Protein Name Transcriptional Repressor Protein Yy1
Delta Transcription Factor
Ino80 Complex Subunit S
Nf-E1
Yin And Yang 1
Yy-1
Database Links Reactome: R-HSA-5617472
Reactome: R-HSA-5689603
Reactome: R-HSA-5696394
Reactome: R-HSA-8866910
Reactome: R-HSA-9018519
Cellular Localisation Nucleus Matrix
Associated With The Nuclear Matrix
Alternative Antibody Names Anti-Transcriptional Repressor Protein Yy1 antibody
Anti-Delta Transcription Factor antibody
Anti-Ino80 Complex Subunit S antibody
Anti-Nf-E1 antibody
Anti-Yin And Yang 1 antibody
Anti-Yy-1 antibody
Anti-YY1 antibody
Anti-INO80S antibody

Information sourced from Uniprot.org

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