• Western blot analysis of lysates from 293 cells, treated with Forskolin 40nM 30', using 14-3-3 zeta Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293T HELA using 14-3-3 Zeta Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
  • Western blot analysis of COLO205 cells using 14-3-3 Zeta Polyclonal Antibody diluted at 1：1000
  • Western blot analysis of various cells using 14-3-3 Zeta Polyclonal Antibody diluted at 1：1000

Anti-YWHAZ antibody (24-73 aa) (STJ91372)

SKU:
STJ91372

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IP/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-14-3-3 protein zeta/delta (24-73 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug mg/lysate
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YWHAZ
Gene ID: 7534
Uniprot ID: 1433Z_HUMAN
Immunogen Region: 24-73 aa
Specificity: 14-3-3 Zeta Polyclonal Antibody detects endogenous levels of 14-3-3 Zeta protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human 14-3-3 zeta at the amino acid range 24-73
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity.
Protein Name 14-3-3 Protein Zeta/Delta
Protein Kinase C Inhibitor Protein 1
Kcip-1
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-3769402
Reactome: R-HSA-392517
Reactome: R-HSA-430116
Reactome: R-HSA-450604
Reactome: R-HSA-512988
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-75035
Reactome: R-HSA-9013700
Reactome: R-HSA-9604323
Reactome: R-HSA-9614399
Reactome: R-HSA-9735871
Reactome: R-HSA-9755779
Cellular Localisation Cytoplasm
Melanosome
Located To Stage I To Stage Iv Melanosomes
Alternative Antibody Names Anti-14-3-3 Protein Zeta/Delta antibody
Anti-Protein Kinase C Inhibitor Protein 1 antibody
Anti-Kcip-1 antibody
Anti-YWHAZ antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance