• Western blot analysis of extracts of various cell lines, using YWHAH antibody (STJ116342) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Anti-YWHAH antibody (1-246) (STJ116342)
  • Immunofluorescence analysis of NIH-3T3 cells using YWHAH Polyclonal Antibody (STJ116342) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using YWHAH Rabbit polyclonal antibody (STJ116342) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using YWHAH Polyclonal Antibody (STJ116342) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using YWHAH Rabbit polyclonal antibody (STJ116342) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-YWHAH antibody (1-246) (STJ116342)

SKU:
STJ116342

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-YWHAH (1-246) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YWHAH
Gene ID: 7533
Uniprot ID: 1433F_HUMAN
Immunogen Region: 1-246
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human YWHAH (NP_003396.1).
Immunogen Sequence: MGDREQLLQRARLAEQAERY DDMASAMKAVTELNEPLSNE DRNLLSVAYKNVVGARRSSW RVISSIEQKTMADGNEKKLE KVKAYREKIEKELETVCNDV LSLLDKFLIKNCNDFQYESK VFYLKMKGDYYRYLAEVASG EKKNSVVEASEAAYKEAFEI SKEQMQPTHPIRLGLALNFS VFYYEIQNAPEQACLLAKQA FDDAIAELDTLNEDSYKDST LIMQLLRDNLTLWTSDQQD
Tissue Specificity Expressed mainly in the brain and present in other tissues albeit at lower levels.
Post Translational Modifications Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Protein Name 14-3-3 Protein Eta
Protein As1
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-75035
Reactome: R-HSA-9735871
Reactome: R-HSA-9755779
Alternative Antibody Names Anti-14-3-3 Protein Eta antibody
Anti-Protein As1 antibody
Anti-YWHAH antibody
Anti-YWHA1 antibody

Information sourced from Uniprot.org

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