• Western blot analysis of extracts of various cell lines, using XPA antibody (STJ26126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
  • Western blot analysis of extracts from normal (control) and XPA knockout (KO) HeLa cells, using XPA antibody (STJ26126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using XPA antibody (STJ26126). Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
  • Immunofluorescence analysis of A549 cells using XPA antibody (STJ26126).

Anti-XPA antibody (1-273) (STJ26126)

SKU:
STJ26126

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XPA
Gene ID: 7507
Uniprot ID: XPA_HUMAN
Immunogen Region: 1-273
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1).
Immunogen Sequence: MAAADGALPEAAALEQPAEL PASVRASIERKRQRALMLRQ ARLAARPYSATAAAATGGMA NVKAAPKIIDTGGGFILEEE EEEEQKIGKVVHQPGPVMEF DYVICEECGKEFMDSYLMNH FDLPTCDNCRDADDKHKLIT KTEAKQEYLLKDCDLEKREP PLKFIVKKNPHHSQWGDMKL YLKLQIVKRSLEVWGSQEAL EEAKEVRQENREKMKQKKFD KKVKELRRAVRSSVWKRET
Tissue Specificity Expressed in various cell lines and in skin fibroblasts.
Post Translational Modifications ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage. Ubiquitinated by HERC2 leading to degradation by the proteasome.
Function Involved in DNA nucleotide excision repair (NER). Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. During NER stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3. Connects XPD/ERCC2 and XPB/ERCC3 during NER, retaining DNA near the XPB/ERCC3 active site, and stabilizing the complex in a different conformation than in transcribing TFIIH.
Protein Name Dna Repair Protein Complementing Xp-A Cells
Xeroderma Pigmentosum Group A-Complementing Protein
Database Links Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-6781823
Reactome: R-HSA-6782135
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Repair Protein Complementing Xp-A Cells antibody
Anti-Xeroderma Pigmentosum Group A-Complementing Protein antibody
Anti-XPA antibody
Anti-XPAC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance