• Western blot analysis of extracts of mouse heart, using SPSB1 antibody (STJ112363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SPSB1 antibody (1-273) (STJ112363)

SKU:
STJ112363

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPSB1 (1-273) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPSB1
Gene ID: 80176
Uniprot ID: SPSB1_HUMAN
Immunogen Region: 1-273
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human SPSB1 (Q96BD6).
Immunogen Sequence: MGQKVTGGIKTVDMRDPTYR PLKQELQGLDYCKPTRLDLL LDMPPVSYDVQLLHSWNNND RSLNVFVKEDDKLIFHRHPV AQSTDAIRGKVGYTRGLHVW QITWAMRQRGTHAVVGVATA DAPLHSVGYTTLVGNNHESW GWDLGRNRLYHDGKNQPSKT YPAFLEPDETFIVPDSFLVA LDMDDGTLSFIVDGQYMGVA FRGLKGKKLYPVVSAVWGHC EIRMRYLNGLDPEPLPLMD
Function Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2. Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5.
Protein Name Spry Domain-Containing Socs Box Protein 1
Ssb-1
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Cytosol
Exhibits A Diffuse Cytosolic Localization
Alternative Antibody Names Anti-Spry Domain-Containing Socs Box Protein 1 antibody
Anti-Ssb-1 antibody
Anti-SPSB1 antibody
Anti-SSB1 antibody

Information sourced from Uniprot.org

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