| Short Description : | Rabbit polyclonal Pre-Mrna-Splicing Regulator Wtap (212-355) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | WTAP |
| Gene ID: | 9589 |
| Uniprot ID: | FL2D_HUMAN |
| Immunogen Region: | 212-355 |
| Immunogen Sequence: | DEEVEGMQSTILVLQQQLKE TRQQLAQYQQQQSQASAPST SRTTASEPVEQSEATSKDCS RLTNGPSNGSSSRQRTSGSG FHREGNTTEDDFPSSPGNGN KSSNSSEERTGRGGSGYVNQ LSAGYESVDSPTGSENSLTH QSND |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 212-355 of human WTAP (NP_004897.2). |
| Tissue Specificity | Ubiquitously expressed. |
| Function | Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. |
| Protein Name | Pre-Mrna-Splicing Regulator WtapFemale-Lethal(2d HomologHfl(2dWt1-Associated ProteinWilms Tumor 1-Associating Protein |
| Database Links | Reactome: R-HSA-72203 |
| Cellular Localisation | Nucleus SpeckleNucleusNucleoplasmCytoplasmMainly Nuclear With Some Fraction Located In The CytoplasmZc3h13 Is Required To Anchor Component Of The Macom SubcomplexSuch As VirmaIn The Nucleus |
| Alternative Antibody Names | Anti-Pre-Mrna-Splicing Regulator Wtap antibodyAnti-Female-Lethal(2d Homolog antibodyAnti-Hfl(2d antibodyAnti-Wt1-Associated Protein antibodyAnti-Wilms Tumor 1-Associating Protein antibodyAnti-WTAP antibodyAnti-KIAA0105 antibody |
Information sourced from Uniprot.org
