| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | WNT5B |
| Gene ID: | 81029 |
| Uniprot ID: | WNT5B_HUMAN |
| Immunogen Region: | 181-342 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human WNT5B (NP_110402.2). |
| Immunogen Sequence: | ASVFGRVMQIGSRETAFTHA VSAAGVVNAISRACREGELS TCGCSRTARPKDLPRDWLWG GCGDNVEYGYRFAKEFVDAR EREKNFAKGSEEQGRVLMNL QNNEAGRRAVYKMADVACKC HGVSGSCSLKTCWLQLAEFR KVGDRLKEKYDSAAAMRVTR KGRLELVNSRFTQPTPEDLV YVDPSPDYCLRNESTGSLGT |
| Post Translational Modifications | Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition. |
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. |
| Protein Name | Protein Wnt-5b |
| Database Links | Reactome: R-HSA-3238698Reactome: R-HSA-4086400 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Protein Wnt-5b antibodyAnti-WNT5B antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and PAK4 knockout (KO) 293T cells, using [KO Validated] PAK4 Rabbit pAb (STJ11100032) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89104/405569/STJ11100032_1__78296.1744891338.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PAK4 knockout (KO) 293T cells, using [KO Validated] PAK4 Rabbit pAb (STJ11100032) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.")
