• Immunohistochemistry analysis of paraffin-embedded human gastric cancer using WNT3A antibody (STJ115562) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using WNT3A antibody (STJ115562) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using WNT3A antibody (STJ115562) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-WNT3A antibody (1-352) (STJ115562)

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STJ115562

£112.50 - £522.50
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Host: Rabbit
Applications: IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WNT3A (1-352) is suitable for use in Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT3A
Gene ID: 89780
Uniprot ID: WNT3A_HUMAN
Immunogen Region: 1-352
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human WNT3A (NP_149122.1).
Immunogen Sequence: MAPLGYFLLLCSLKQALGSY PIWWSLAVGPQYSSLGSQPI LCASIPGLVPKQLRFCRNYV EIMPSVAEGIKIGIQECQHQ FRGRRWNCTTVHDSLAIFGP VLDKATRESAFVHAIASAGV AFAVTRSCAEGTAAICGCSS RHQGSPGKGWKWGGCSEDIE FGGMVSREFADARENRPDAR SAMNRHNNEAGRQAIASHMH LKCKCHGLSGSCEVKTCWWS QPDFRAIGDFLKDKYDSAS
Tissue Specificity Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
Post Translational Modifications Palmitoleoylation by PORCN is required for efficient binding to frizzled receptors. Palmitoleoylation is required for proper trafficking to cell surface, vacuolar acidification is critical to release palmitoleoylated WNT3A from WLS in secretory vesicles. Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway, possibly by promoting disulfide bond formation and oligomerization. Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT3A. Disulfide bonds have critical and distinct roles in secretion and activity. Loss of each conserved cysteine in WNT3A results in high molecular weight oxidized Wnt oligomers, which are formed through inter-Wnt disulfide bonding.
Function Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
Protein Name Protein Wnt-3a
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Reactome: R-HSA-3772470
Reactome: R-HSA-4641262
Reactome: R-HSA-4641263
Reactome: R-HSA-5340588
Reactome: R-HSA-9793380
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-3a antibody
Anti-WNT3A antibody

Information sourced from Uniprot.org

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