• Immunohistochemistry analysis of WIPF1 in paraffin-embedded mouse spleen using WIPF1 Rabbit polyclonal antibody (STJ11102826) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of WIPF1 in paraffin-embedded human esophageal using WIPF1 Rabbit polyclonal antibody (STJ11102826) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of WIPF1 in paraffin-embedded human appendix using WIPF1 Rabbit polyclonal antibody (STJ11102826) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using WIPF1 Rabbit polyclonal antibody (STJ11102826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WIPF1 antibody (400-500) (STJ11102826)

SKU:
STJ11102826

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WIPF1 (400-500) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WIPF1
Gene ID: 7456
Uniprot ID: WIPF1_HUMAN
Immunogen Region: 400-500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human WIPF1 (NP_001070737.1).
Immunogen Sequence: QLPSRSGVDSPRSGPRPPLP PDRPSAGAPPPPPPSTSIRN GFQDSPCEDEWESRFYFHPI SDLPPPEPYVQTTKSYPSKL ARNESRSGSNRRERGAPPLP P
Tissue Specificity Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
Function Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles. Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.
Protein Name Was/Wasl-Interacting Protein Family Member 1
Protein Prpl-2
Wiskott-Aldrich Syndrome Protein-Interacting Protein
Wasp-Interacting Protein
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-5663213
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasmic Vesicle
Cytoplasm
Cytoskeleton
Cell Projection
Ruffle
Vesicle Surfaces And Along Actin Tails
Colocalizes With Actin Stress Fibers
When Coexpressed With Wasl
No Longer Associated With Actin Filaments But Accumulated In Perinuclear And Cortical Areas Like Wasl
Alternative Antibody Names Anti-Was/Wasl-Interacting Protein Family Member 1 antibody
Anti-Protein Prpl-2 antibody
Anti-Wiskott-Aldrich Syndrome Protein-Interacting Protein antibody
Anti-Wasp-Interacting Protein antibody
Anti-WIPF1 antibody
Anti-WASPIP antibody
Anti-WIP antibody

Information sourced from Uniprot.org

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