• Western blot analysis of extracts of various cell lines, using WIF1 Rabbit monoclonal antibody (STJ11102282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-WIF1 antibody (280-379) [S2MR] (STJ11102282)

SKU:
STJ11102282

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-WIF1 (280-379) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WIF1
Gene ID: 11197
Uniprot ID: WIF1_HUMAN
Immunogen Region: 280-379
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 280-379 of human WIF1 (Q9Y5W5).
Immunogen Sequence: QPCRNGGKCIGKSKCKCSKG YQGDLCSKPVCEPGCGAHGT CHEPNKCQCQEGWHGRHCNK RYEASLIHALRPAGAQLRQH TPSLKKAEERRDPPESNYIW
Function Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Protein Name Wnt Inhibitory Factor 1
Wif-1
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Cellular Localisation Secreted
Alternative Antibody Names Anti-Wnt Inhibitory Factor 1 antibody
Anti-Wif-1 antibody
Anti-WIF1 antibody
Anti-UNQ191 antibody
Anti-PRO217 antibody

Information sourced from Uniprot.org

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