| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WDR77 (153-243) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WDR77 |
| Gene ID: |
79084 |
| Uniprot ID: |
MEP50_HUMAN |
| Immunogen Region: |
153-243 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 153-243 of human WDR77 (NP_077007.1). |
| Immunogen Sequence: |
DLAQQVVLSSYRAHAAQVTC VAASPHKDSVFLSCSEDNRI LLWDTRCPKPASQIGCSAPG YLPTSLAWHPQQSEVFVFGD ENGTVSLVDTK |
| Tissue Specificity | Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors. |
| Function | Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. |
| Protein Name | Methylosome Protein Wdr77Androgen Receptor Cofactor P44Methylosome Protein 50Mep-50Wd Repeat-Containing Protein 77P44/Mep50 |
| Database Links | Reactome: R-HSA-191859Reactome: R-HSA-3214858 |
| Cellular Localisation | NucleusCytoplasmNuclear In Leydig Cells And Cytoplasmic In Germ Cells During Fetal Testicular DevelopmentIn Adult TestisPredominantly NuclearSubcellular Location Varies From Nuclear To Cytoplasmic In Various Tumors |
| Alternative Antibody Names | Anti-Methylosome Protein Wdr77 antibodyAnti-Androgen Receptor Cofactor P44 antibodyAnti-Methylosome Protein 50 antibodyAnti-Mep-50 antibodyAnti-Wd Repeat-Containing Protein 77 antibodyAnti-P44/Mep50 antibodyAnti-WDR77 antibodyAnti-MEP50 antibodyAnti-WD45 antibodyAnti-HKMT1069 antibodyAnti-Nbla10071 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
