• Immunofluorescence analysis of NIH/3T3 cells using WDR6 Rabbit polyclonal antibody (STJ110090) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from SH-SY5Y cells, using WDR6 Rabbit polyclonal antibody (STJ110090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-WDR6 antibody (800-1000) (STJ110090)

SKU:
STJ110090

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WDR6 (800-1000) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WDR6
Gene ID: 11180
Uniprot ID: WDR6_HUMAN
Immunogen Region: 800-1000
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 800-1000 of human WDR6 (NP_060501.3).
Immunogen Sequence: VCNHISSVRAVAVWGIGTPG GPQDPQPGLTAHVVSAGGRA EMHCFSIMVTPDPSTPSRLA CHVMHLSSHRLDEYWDRQRN RHRMVKVDPETRYMSLAVCE LDQPGLGPLVAAACSDGAVR LFLLQDSGRILQLLAETFHH KRCVLKVHSFTHEAPNQRRR LLLCSAATDGSLAFWDLTTM LDHDSTVLEPPVDPGLPYRL G
Tissue Specificity Ubiquitous.
Function Together with methyltransferase FTSJ1, methylates the 2'-O-ribose of nucleotides at position 34 of the tRNA anticodon loop of substrate tRNAs. Required for the correct positioning of the substrate tRNA for methylation. Required to suppress amino acid starvation-induced autophagy. Enhances the STK11/LKB1-induced cell growth suppression activity.
Protein Name Trna
34-2'-O-Methyltransferase Regulator Wdr6
Wd Repeat-Containing Protein 6
Database Links Reactome: R-HSA-9013420
Reactome: R-HSA-9013424
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Cellular Localisation Cytoplasm
Colocalizes In The Cytoplasm With Stk11/Lkb1
Alternative Antibody Names Anti-Trna antibody
Anti-34-2'-O-Methyltransferase Regulator Wdr6 antibody
Anti-Wd Repeat-Containing Protein 6 antibody
Anti-WDR6 antibody

Information sourced from Uniprot.org

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