• Immunofluorescence analysis of U2OS cells using WBP11 Rabbit polyclonal antibody (STJ118631) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using WBP11 Rabbit polyclonal antibody (STJ118631) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using WBP11 Rabbit polyclonal antibody (STJ118631) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using WBP11 Rabbit polyclonal antibody (STJ118631) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using WBP11 Rabbit polyclonal antibody (STJ118631) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using WBP11 antibody (STJ118631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-WBP11 antibody (540-641) (STJ118631)

SKU:
STJ118631

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WBP11 (540-641) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WBP11
Gene ID: 51729
Uniprot ID: WBP11_HUMAN
Immunogen Region: 540-641
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 540-641 of human WBP11 (NP_057396.1).
Immunogen Sequence: LIQRPKADDTSAATIEKKAT ATISAKPQITNPKAEITRFV PTALRVRRENKGATAAPQRK SEDDSAVPLAKAAPKSGPSV PVSVQTKDDVYEAFMKEMEG LL
Tissue Specificity Ubiquitous. Highly expressed in the heart, pancreas, kidney skeletal muscle, placenta and brain (at protein level). Weakly expressed in liver and lung.
Function Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity.
Protein Name Ww Domain-Binding Protein 11
Wbp-11
Npw38-Binding Protein
Npwbp
Sh3 Domain-Binding Protein Snp70
Splicing Factor That Interacts With Pqbp-1 And Pp1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Cytoplasm
Predominantly Located In The Nucleus With Granular Heterogeneous Distribution
Excluded From Nucleoli In Interphase Cells
Distributed Throughout Cytoplasm In Dividing Cells
Colocalized With Sc35 And U2b In The Nucleus
In The Cytoplasm
Associates With The Intermediate Filament Protein Vimentin
Alternative Antibody Names Anti-Ww Domain-Binding Protein 11 antibody
Anti-Wbp-11 antibody
Anti-Npw38-Binding Protein antibody
Anti-Npwbp antibody
Anti-Sh3 Domain-Binding Protein Snp70 antibody
Anti-Splicing Factor That Interacts With Pqbp-1 And Pp1 antibody
Anti-WBP11 antibody
Anti-NPWBP antibody
Anti-SIPP1 antibody
Anti-SNP70 antibody

Information sourced from Uniprot.org

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