| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | VPS37A |
| Gene ID: | 137492 |
| Uniprot ID: | VP37A_HUMAN |
| Immunogen Region: | 224-397 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 224-397 of human VPS37A (NP_689628.2). |
| Immunogen Sequence: | MPDVPDAFPELSELSVSQLT DMNEQEEVLLEQFLTLPQLK QIITDKDDLVKSIEELARKN LLLEPSLEAKRQTVLDKYEL LTQMKSTFEKKMQRQHELSE SCSASALQARLKVAAHEAEE ESDNIAEDFLEGKMEIDDFL SSFMEKRTICHCRRAKEEKL QQAIAMHSQFHAPL |
| Tissue Specificity | Widely expressed. Examined tissues include heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. More abundant in liver. Strongly decreased or undetected in hepatomas. |
| Function | Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 37aHvps37aEscrt-I Complex Subunit Vps37aHepatocellular Carcinoma-Related Protein 1 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-174490Reactome: R-HSA-917729Reactome: R-HSA-9610379Reactome: R-HSA-9615710 |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinNucleus |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 37a antibodyAnti-Hvps37a antibodyAnti-Escrt-I Complex Subunit Vps37a antibodyAnti-Hepatocellular Carcinoma-Related Protein 1 antibodyAnti-VPS37A antibodyAnti-HCRP1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and SETDB1 knockout (KO) 293T cells, using [KO Validated] SETDB1 Rabbit pAb (STJ27898) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/101387/411297/STJ27898_1__64075.1744897831.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SETDB1 knockout (KO) 293T cells, using [KO Validated] SETDB1 Rabbit pAb (STJ27898) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.")
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ113207) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/92785/373370/STJ113207_1__86638.1713139598.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ113207) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
