Host: | Goat |
Applications: | Pep-ELISA/WB |
Reactivity: | Human/Dog/Cow |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Goat polyclonal antibody anti-VLCAD (C-Term) is suitable for use in ELISA and Western Blot research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 70kDa band observed in Human Heart lysates (calculated MW of 70.4kDa according to NP_000009.1). Recommended concentration: 1-3µg/ml. |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | ACADVL |
Gene ID: | 37 |
Uniprot ID: | ACADV_HUMAN |
Immunogen Region: | C-Term |
Accession Number: | NP_000009.1; NP_001029031.1 |
Specificity: | This antibody is expected to recognize both reported isoforms (NP_000009.1; NP_001029031.1). |
Immunogen Sequence: | RSLSEGHPTAQHE |
Post Translational Modifications | S-nitrosylation at Cys-237 in liver improves catalytic efficiency. |
Function | Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains. |
Protein Name | Very Long-Chain Specific Acyl-Coa Dehydrogenase - MitochondrialVlcad |
Database Links | Reactome: R-HSA-381038Reactome: R-HSA-77305 |
Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinIsoform 2: Mitochondrion Inner Membrane |
Alternative Antibody Names | Anti-Very Long-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibodyAnti-Vlcad antibodyAnti-ACADVL antibodyAnti-VLCAD antibody |
Information sourced from Uniprot.org