Western blot analysis of extracts of Raji cells, using VIPR1 antibody (STJ116410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-VIPR1 antibody (31-150) (STJ116410)

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STJ116410
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VIPR1
Gene ID: 7433
Uniprot ID: VIPR1_HUMAN
Immunogen Region: 31-150
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 31-150 of human VIPR1 (NP_004615.2).
Immunogen Sequence: ARLQEECDYVQMIEVQHKQC LEEAQLENETIGCSKMWDNL TCWPATPRGQVVVLACPLIF KLFSSIQGRNVSRSCTDEGW THLEPGPYPIACGLDDKAAS LDEQQTMFYGSVKTGYTIGY
Tissue Specificity In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T-cell lines Peer, MOLT-4, HSB and YT.
Function This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38.
Protein Name Vasoactive Intestinal Polypeptide Receptor 1
Vip-R-1
Pituitary Adenylate Cyclase-Activating Polypeptide Type Ii Receptor
Pacap Type Ii Receptor
Pacap-R-2
Pacap-R2
Vpac1
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-420092
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Vasoactive Intestinal Polypeptide Receptor 1 antibody
Anti-Vip-R-1 antibody
Anti-Pituitary Adenylate Cyclase-Activating Polypeptide Type Ii Receptor antibody
Anti-Pacap Type Ii Receptor antibody
Anti-Pacap-R-2 antibody
Anti-Pacap-R2 antibody
Anti-Vpac1 antibody
Anti-VIPR1 antibody

Information sourced from Uniprot.org

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