• Immunofluorescence analysis of L929 cells using USP5 antibody (STJ26063) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using USP5 antibody (STJ26063) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using USP5 Rabbit polyclonal antibody (STJ26063) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using USP5 Rabbit polyclonal antibody (STJ26063) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using USP5 Rabbit polyclonal antibody (STJ26063) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat stomach using USP5 Rabbit polyclonal antibody (STJ26063) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using USP5 antibody (STJ26063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-USP5 antibody (1-280) (STJ26063)

SKU:
STJ26063

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP5
Gene ID: 8078
Uniprot ID: UBP5_HUMAN
Immunogen Region: 1-280
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP5 (NP_003472.2).
Immunogen Sequence: MAELSEEALLSVLPTIRVPK AGDRVHKDECAFSFDTPESE GGLYICMNTFLGFGKQYVER HFNKTGQRVYLHLRRTRRPK EEDPATGTGDPPRKKPTRLA IGVEGGFDLSEEKFELDEDV KIVILPDYLEIARDGLGGLP DIVRDRVTSAVEALLSADSA SRKQEVQAWDGEVRQVSKHA FSLKQLDNPARIPPCGWKCS KCDMRENLWLNLTDGSILCG RRYFDGSGGNNHAVEHYRE
Function Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 5
Deubiquitinating Enzyme 5
Isopeptidase T
Ubiquitin Thioesterase 5
Ubiquitin-Specific-Processing Protease 5
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-8866652
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 5 antibody
Anti-Deubiquitinating Enzyme 5 antibody
Anti-Isopeptidase T antibody
Anti-Ubiquitin Thioesterase 5 antibody
Anti-Ubiquitin-Specific-Processing Protease 5 antibody
Anti-USP5 antibody
Anti-ISOT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance