Anti-USP30 antibody (57-390) (STJ114728)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal USP30 (57-390) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP30 |
| Gene ID | 84749 |
| Uniprot ID | UBP30_HUMAN |
| Immunogen Region | 57-390 |
| Immunogen Sequence | TERKKRRKGLVPGLVNLGNT CFMNSLLQGLSACPAFIRWL EEFTSQYSRDQKEPPSHQYL SLTLLHLLKALSCQEVTDDE VLDASCLLDVLRMYRWQISS FEEQDAHELFHVITSSLEDE RDRQPRVTHLFDVHSLEQQS EITPKQITCRTRGSPHPTSN HWKSQHPFHGRLTSNMVCKH CEHQSPVRFDTFDSLSLSIP AATWGHPLTLDHCLHHFISS ESVRDVVCDNCTKIEAKGT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 57-390 of human USP30 (NP_116052.2). |
Additional Info
| Tissue Specificity | Expressed in skeletal muscle, pancreas, liver and kidney. |
| Post Translational Modifications | Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation. |
| Function | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as a negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 30Deubiquitinating Enzyme 30Ubiquitin Thioesterase 30Ubiquitin-Specific-Processing Protease 30Ub-Specific Protease 30 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-9664873 |
| Cellular Localisation | Mitochondrion Outer Membrane |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 30 antibodyAnti-Deubiquitinating Enzyme 30 antibodyAnti-Ubiquitin Thioesterase 30 antibodyAnti-Ubiquitin-Specific-Processing Protease 30 antibodyAnti-Ub-Specific Protease 30 antibodyAnti-USP30 antibody |
Information sourced from Uniprot.org