| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-USP25 (1-230) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
USP25 |
| Gene ID: |
29761 |
| Uniprot ID: |
UBP25_HUMAN |
| Immunogen Region: |
1-230 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_037528.3). |
| Immunogen Sequence: |
MTVEQNVLQQSAAQKHQQTF LNQLREITGINDTQILQQAL KDSNGNLELAVAFLTAKNAK TPQQEETTYYQTALPGNDRY ISVGSQADTNVIDLTGDDKD DLQRAIALSLAESNRAFRET GITDEEQAISRVLEASIAEN KACLKRTPTEVWRDSRNPYD RKRQDKAPVGLKNVGNTCWF SAVIQSLFNLLEFRRLVLNY KPPSNAQDLPRNQKEHRNLP FMRELRYLFA |
| Tissue Specificity | Isoform USP25a is found in most adult and fetal tissues.expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific. |
| Post Translational Modifications | Acetylated. Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue. Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions. Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels. |
| Function | Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 25Deubiquitinating Enzyme 25Usp On Chromosome 21Ubiquitin Thioesterase 25Ubiquitin-Specific-Processing Protease 25 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | CytoplasmIsoform Usp25m: CytoplasmNucleusSome Transient Punctuate Nuclear Location In Myotubes During Myocyte Development |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 25 antibodyAnti-Deubiquitinating Enzyme 25 antibodyAnti-Usp On Chromosome 21 antibodyAnti-Ubiquitin Thioesterase 25 antibodyAnti-Ubiquitin-Specific-Processing Protease 25 antibodyAnti-USP25 antibodyAnti-USP21 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
