• Immunofluorescence analysis of U2OS cells using USP11 Rabbit polyclonal antibody (STJ11103210) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat testis, using USP11 Rabbit polyclonal antibody (STJ11103210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using USP11 Rabbit polyclonal antibody (STJ11103210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-USP11 antibody (70-190) (STJ11103210)

SKU:
STJ11103210

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP11 (70-190) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP11Uniprot ID=UBP11_HUMAN"
Immunogen Region: 70-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 70-190 of human USP11 (NP_004642.2).
Immunogen Sequence: PQHEELPGLDSQWRQIENGE SGRERPLRAGESWFLVEKHW YKQWEAYVQGGDQDSSTFPG CINNATLFQDEINWRLKEGL VEGEDYVLLPAAAWHYLVSW YGLEHGQPPIERKVIELPNI Q
Function Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro). Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks. Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex.may act by deubiquitinating components of the PRC1-like complex. Promotes cell proliferation by deubiquitinating phosphorylated E2F1.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 11
Deubiquitinating Enzyme 11
Ubiquitin Thioesterase 11
Ubiquitin-Specific-Processing Protease 11
Cellular Localisation Nucleus
Cytoplasm
Chromosome
Predominantly Nuclear
Associates With Chromatin
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 11 antibody
Anti-Deubiquitinating Enzyme 11 antibody
Anti-Ubiquitin Thioesterase 11 antibody
Anti-Ubiquitin-Specific-Processing Protease 11 antibody
Anti-USP11 antibody
Anti-UHX1 antibody

Information sourced from Uniprot.org

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