• STJ70512 (0. 1µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70512 (10µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. (Data have been obtained with STJ70512 from a previous batch).

Anti-USH1C/Harmonin antibody (N-Term) (STJ70512)

SKU:
STJ70512-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-USH1C/Harmonin (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: USH1C
Gene ID: 10083
Uniprot ID: USH1C_HUMAN
Immunogen Region: N-Term
Accession Number: NP_005700.2; NP_710142.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_005700.2 and NP_710142.1).
Immunogen Sequence: DRKVAREFRHKVD
Function Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal development and maintenance of cochlear hair cell bundles. As part of the intermicrovillar adhesion complex/IMAC plays a role in brush border differentiation, controlling microvilli organization and length. Probably plays a central regulatory role in the assembly of the complex, recruiting CDHR2, CDHR5 and MYO7B to the microvilli tips.
Protein Name Harmonin
Antigen Ny-Co-38/Ny-Co-37
Autoimmune Enteropathy-Related Antigen Aie-75
Protein Pdz-73
Renal Carcinoma Antigen Ny-Ren-3
Usher Syndrome Type-1c Protein
Database Links Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Cell Projection
Microvillus
Colocalizes With F-Actin
Detected At The Tip Of Cochlear Hair Cell Stereocilia
Enriched In Microvilli Of The Intestinal Brush Border
Alternative Antibody Names Anti-Harmonin antibody
Anti-Antigen Ny-Co-38/Ny-Co-37 antibody
Anti-Autoimmune Enteropathy-Related Antigen Aie-75 antibody
Anti-Protein Pdz-73 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-3 antibody
Anti-Usher Syndrome Type-1c Protein antibody
Anti-USH1C antibody
Anti-AIE75 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance