• Immunofluorescence analysis of L929 cells using UPF3A Rabbit polyclonal antibody (STJ118338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using UPF3A Rabbit polyclonal antibody (STJ118338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using UPF3A Rabbit polyclonal antibody (STJ118338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from mouse testis, using UPF3A Rabbit polyclonal antibody (STJ118338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-UPF3A antibody (327-476) (STJ118338)

SKU:
STJ118338

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UPF3A (327-476) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UPF3A
Gene ID: 65110
Uniprot ID: REN3A_HUMAN
Immunogen Region: 327-476
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 327-476 of human UPF3A (NP_075387.1).
Immunogen Sequence: EGSLEEPQETSHSGSDKEHR DVERSQEQESEAQRYHVDDG RRHRAHHEPERLSRRSEDEQ RWGKGPGQDRGKKGSQDSGA PGEAMERLGRAQRCDDSPAP RKERLANKDRPALQLYDPGA RFRARECGGNRRICKAEGSG TGPEKREEAE
Tissue Specificity Isoform 1 is strongly expressed in testis, uterus, muscle, fetal brain and spinal cord. Isoform 2 is strongly expressed in fetal brain and spinal cord.
Function Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation.
Protein Name Regulator Of Nonsense Transcripts 3a
Nonsense Mrna Reducing Factor 3a
Up-Frameshift Suppressor 3 Homolog A
Hupf3
Database Links Reactome: R-HSA-9010553
Reactome: R-HSA-975957 Q9H1J1-2
Cellular Localisation Nucleus
Cytoplasm
Shuttling Between The Nucleus And The Cytoplasm
Alternative Antibody Names Anti-Regulator Of Nonsense Transcripts 3a antibody
Anti-Nonsense Mrna Reducing Factor 3a antibody
Anti-Up-Frameshift Suppressor 3 Homolog A antibody
Anti-Hupf3 antibody
Anti-UPF3A antibody
Anti-RENT3A antibody
Anti-UPF3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance