• Western blot analysis of various lysates, using UPF2 rabbit polyclonal antibody (STJ115373) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using UPF2 antibody (STJ115373) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug UPF2 rabbit polyclonal antibody (STJ115373 1:200). Western blot was performed from the immunoprecipitate using UPF2 rabbit polyclonal antibody (A7091) at a dilition of 1:1000.

Anti-UPF2 antibody (1153-1272) (STJ115373)

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STJ115373

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UPF2 (1153-1272) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UPF2
Gene ID: 26019
Uniprot ID: RENT2_HUMAN
Immunogen Region: 1153-1272
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_542166.1).
Immunogen Sequence: GPPLGGGEGEAESADTMPFV MLTRKGNKQQFKILNVPMSS QLAANHWNQQQAEQEERMRM KKLTLDINERQEQEDYQEML QSLAQRPAPANTNRERRPRY QHPKGAPNADLIFKTGGRRR
Tissue Specificity Ubiquitous.
Function Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA.
Protein Name Regulator Of Nonsense Transcripts 2
Up-Frameshift Suppressor 2 Homolog
Hupf2
Database Links Reactome: R-HSA-9010553
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Regulator Of Nonsense Transcripts 2 antibody
Anti-Up-Frameshift Suppressor 2 Homolog antibody
Anti-Hupf2 antibody
Anti-UPF2 antibody
Anti-KIAA1408 antibody
Anti-RENT2 antibody

Information sourced from Uniprot.org

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