• Confocal imaging of C2C12 cells using ULK3 Rabbit monoclonal antibody (STJ11103598, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of lysates from 293T cells using ULK3 Rabbit monoclonal antibody (STJ11103598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-ULK3 antibody (373-472) [S8MR] (STJ11103598)

SKU:
STJ11103598

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ULK3 (373-472) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ULK3
Gene ID: 25989
Uniprot ID: ULK3_HUMAN
Immunogen Region: 373-472
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 373-472 of human ULK3 (Q6PHR2).
Immunogen Sequence: KPRLLAALEVASAAMAKEEA AGGEQDALDLYQHSLGELLL LLAAEPPGRRRELLHTEVQN LMARAEYLKEQVKMRESRWE ADTLDKEGLSESVRSSCTLQ
Tissue Specificity Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.
Post Translational Modifications Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU.
Function Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
Protein Name Serine/Threonine-Protein Kinase Ulk3
Unc-51-Like Kinase 3
Database Links Reactome: R-HSA-5632684
Cellular Localisation Cytoplasm
Localizes To Pre-Autophagosomal Structure During Cellular Senescence
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Ulk3 antibody
Anti-Unc-51-Like Kinase 3 antibody
Anti-ULK3 antibody

Information sourced from Uniprot.org

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