• Immunofluorescence analysis of paraffin-embedded rat brown adipose using UCP1 Rabbit polyclonal antibody (STJ28420) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of UCP1 in paraffin-embedded rat brown adipose using UCP1 Rabbit polyclonal antibody (STJ28420) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse brown adipose, using UCP1 Rabbit polyclonal antibody (STJ28420) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-UCP1 antibody (239-253) (STJ28420)

SKU:
STJ28420

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UCP1 (239-253) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UCP1
Gene ID: 7350
Uniprot ID: UCP1_HUMAN
Immunogen Region: 239-253
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 239-253 of human UCP1 (NP_068605.1).
Immunogen Sequence: RFINSPPGQYKSVPN
Tissue Specificity Brown adipose tissue.
Post Translational Modifications May undergo sulfenylation upon cold exposure. May increase the sensitivity of UCP1 thermogenic function to the activation by noradrenaline probably through structural effects. May undergo ubiquitin-mediated proteasomal degradation.
Function Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria.
Protein Name Mitochondrial Brown Fat Uncoupling Protein 1
Ucp 1
Solute Carrier Family 25 Member 7
Thermogenin
Database Links Reactome: R-HSA-167826
Reactome: R-HSA-167827
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Brown Fat Uncoupling Protein 1 antibody
Anti-Ucp 1 antibody
Anti-Solute Carrier Family 25 Member 7 antibody
Anti-Thermogenin antibody
Anti-UCP1 antibody
Anti-SLC25A7 antibody
Anti-UCP antibody

Information sourced from Uniprot.org

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