• Immunofluorescence analysis of A549 cells using UBE2R2 antibody (STJ29510).
  • Western blot analysis of extracts of various cell lines, using UBE2R2 antibody (STJ29510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-UBE2R2 antibody (1-238) (STJ29510)

SKU:
STJ29510

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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2R2
Gene ID: 54926
Uniprot ID: UB2R2_HUMAN
Immunogen Region: 1-238
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of human UBE2R2 (NP_060281.2).
Immunogen Sequence: MAQQQMTSSQKALMLELKSL QEEPVEGFRITLVDESDLYN WEVAIFGPPNTLYEGGYFKA HIKFPIDYPYSPPTFRFLTK MWHPNIYENGDVCISILHPP VDDPQSGELPSERWNPTQNV RTILLSVISLLNEPNTFSPA NVDASVMFRKWRDSKGKDKE YAEIIRKQVSATKAEAEKDG VKVPTTLAEYCIKTKVPSND NSSDLLYDDLYDDDIDDEDE EEEDADCYDDDDSGNEES
Function E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. Works in collaboration with various Cul1-RING and Cul2-RING E3 ligase complexes. May be involved in degradation of katenin.
Protein Name Ubiquitin-Conjugating Enzyme E2 R2
E2 Ubiquitin-Conjugating Enzyme R2
Ubiquitin Carrier Protein R2
Ubiquitin-Conjugating Enzyme E2-Cdc34b
Ubiquitin-Protein Ligase R2
Database Links Reactome: R-HSA-8866652
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 R2 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme R2 antibody
Anti-Ubiquitin Carrier Protein R2 antibody
Anti-Ubiquitin-Conjugating Enzyme E2-Cdc34b antibody
Anti-Ubiquitin-Protein Ligase R2 antibody
Anti-UBE2R2 antibody
Anti-CDC34B antibody
Anti-UBC3B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance