| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S9MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBE2I |
| Gene ID: | 7329 |
| Uniprot ID: | UBC9_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2I (P63279). |
| Immunogen Sequence: | MSGIALSRLAQERKAWRKDH PFGFVAVPTKNPDGTMNLMN WECAIPGKKGTPWEGGLFKL RMLFKDDYPSSPPKCKFEPP LFHPNVYPSGTVCLSILEED |
| Tissue Specificity | Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. |
| Post Translational Modifications | Phosphorylation at Ser-71 significantly enhances SUMOylation activity. |
| Function | Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity. |
| Protein Name | Sumo-Conjugating Enzyme Ubc9Ring-Type E3 Sumo Transferase Ubc9Sumo-Protein LigaseUbiquitin Carrier Protein 9Ubiquitin Carrier Protein IUbiquitin-Conjugating Enzyme E2 IUbiquitin-Protein Ligase IP18 |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-196791Reactome: R-HSA-3065678Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-3232142Reactome: R-HSA-3899300Reactome: R-HSA-4085377Reactome: R-HSA-4090294Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-4655427Reactome: R-HSA-4755510Reactome: R-HSA-5693565Reactome: R-HSA-5693607Reactome: R-HSA-5696395Reactome: R-HSA-8866904Reactome: R-HSA-9615933Reactome: R-HSA-9683610Reactome: R-HSA-9694631Reactome: R-HSA-9735871Reactome: R-HSA-9793242Reactome: R-HSA-9833482Reactome: R-HSA-9843940 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionMainly NuclearIn SpermatocytesLocalizes In Synaptonemal ComplexesRecruited By Bcl11a Into The Nuclear Body |
| Alternative Antibody Names | Anti-Sumo-Conjugating Enzyme Ubc9 antibodyAnti-Ring-Type E3 Sumo Transferase Ubc9 antibodyAnti-Sumo-Protein Ligase antibodyAnti-Ubiquitin Carrier Protein 9 antibodyAnti-Ubiquitin Carrier Protein I antibodyAnti-Ubiquitin-Conjugating Enzyme E2 I antibodyAnti-Ubiquitin-Protein Ligase I antibodyAnti-P18 antibodyAnti-UBE2I antibodyAnti-UBC9 antibodyAnti-UBCE9 antibody |
Information sourced from Uniprot.org
![Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at a dilution of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/90674/360687/STJ11101759_1__34437.1713125386.jpg?c=1 "Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at a dilution of 1:1000.")
