• Western blot analysis of extracts of various cell lines, using UBE2I rabbit monoclonal antibody (STJ11102069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of HeLa cells, using UBE2I rabbit monoclonal antibody (STJ11102069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of UBE2I in paraffin-embedded human thyroid cancer tissue using UBE2I rabbit monoclonal antibody (STJ11102069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2I in paraffin-embedded mouse brain tissue using UBE2I rabbit monoclonal antibody (STJ11102069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2I in paraffin-embedded mouse lung tissue using UBE2I rabbit monoclonal antibody (STJ11102069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2I in paraffin-embedded mouse spleen tissue using UBE2I rabbit monoclonal antibody (STJ11102069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2I in paraffin-embedded mouse testis tissue using UBE2I rabbit monoclonal antibody (STJ11102069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2I in paraffin-embedded rat brain tissue using UBE2I rabbit monoclonal antibody (STJ11102069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2I in paraffin-embedded rat colon tissue using UBE2I rabbit monoclonal antibody (STJ11102069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-UBE2I antibody (1-100) [S9MR] (STJ11102069)

SKU:
STJ11102069

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-UBE2I (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2I
Gene ID: 7329
Uniprot ID: UBC9_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2I (P63279).
Immunogen Sequence: MSGIALSRLAQERKAWRKDH PFGFVAVPTKNPDGTMNLMN WECAIPGKKGTPWEGGLFKL RMLFKDDYPSSPPKCKFEPP LFHPNVYPSGTVCLSILEED
Tissue Specificity Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Post Translational Modifications Phosphorylation at Ser-71 significantly enhances SUMOylation activity.
Function Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.
Protein Name Sumo-Conjugating Enzyme Ubc9
Ring-Type E3 Sumo Transferase Ubc9
Sumo-Protein Ligase
Ubiquitin Carrier Protein 9
Ubiquitin Carrier Protein I
Ubiquitin-Conjugating Enzyme E2 I
Ubiquitin-Protein Ligase I
P18
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-196791
Reactome: R-HSA-3065678
Reactome: R-HSA-3108214
Reactome: R-HSA-3232118
Reactome: R-HSA-3232142
Reactome: R-HSA-3899300
Reactome: R-HSA-4085377
Reactome: R-HSA-4090294
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-4655427
Reactome: R-HSA-4755510
Reactome: R-HSA-5693565
Reactome: R-HSA-5693607
Reactome: R-HSA-5696395
Reactome: R-HSA-8866904
Reactome: R-HSA-9615933
Reactome: R-HSA-9683610
Reactome: R-HSA-9694631
Reactome: R-HSA-9735871
Reactome: R-HSA-9793242
Cellular Localisation Nucleus
Cytoplasm
Perinuclear Region
Mainly Nuclear
In Spermatocytes
Localizes In Synaptonemal Complexes
Recruited By Bcl11a Into The Nuclear Body
Alternative Antibody Names Anti-Sumo-Conjugating Enzyme Ubc9 antibody
Anti-Ring-Type E3 Sumo Transferase Ubc9 antibody
Anti-Sumo-Protein Ligase antibody
Anti-Ubiquitin Carrier Protein 9 antibody
Anti-Ubiquitin Carrier Protein I antibody
Anti-Ubiquitin-Conjugating Enzyme E2 I antibody
Anti-Ubiquitin-Protein Ligase I antibody
Anti-P18 antibody
Anti-UBE2I antibody
Anti-UBC9 antibody
Anti-UBCE9 antibody

Information sourced from Uniprot.org

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