• STJ70226 (2µg/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70226 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic/Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70226 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70226 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-UBE2C/UBCH10 antibody (C-Term) (STJ70226)

SKU:
STJ70226-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-UBE2C/UBCH10 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: UBE2C
Gene ID: 11065
Uniprot ID: UBE2C_HUMAN
Immunogen Region: C-Term
Accession Number: NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1; NP_001268670.1
Specificity: This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
Immunogen Sequence: QETYSKQVTSQEP
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Protein Name Ubiquitin-Conjugating Enzyme E2 C
(E3-Independent E2 Ubiquitin-Conjugating Enzyme C
E2 Ubiquitin-Conjugating Enzyme C
Ubch10
Ubiquitin Carrier Protein C
Ubiquitin-Protein Ligase C
Database Links Reactome: R-HSA-141430
Reactome: R-HSA-174048
Reactome: R-HSA-174084
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-176407
Reactome: R-HSA-176408
Reactome: R-HSA-176409
Reactome: R-HSA-176412
Reactome: R-HSA-179409
Reactome: R-HSA-2467813
Reactome: R-HSA-2559582
Reactome: R-HSA-68867
Reactome: R-HSA-69017
Reactome: R-HSA-8853884
Reactome: R-HSA-8866652
Reactome: R-HSA-9687136
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 C antibody
Anti-(E3-Independent E2 Ubiquitin-Conjugating Enzyme C antibody
Anti-E2 Ubiquitin-Conjugating Enzyme C antibody
Anti-Ubch10 antibody
Anti-Ubiquitin Carrier Protein C antibody
Anti-Ubiquitin-Protein Ligase C antibody
Anti-UBE2C antibody
Anti-UBCH10 antibody

Information sourced from Uniprot.org

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