• Western blot analysis of extracts of various cell lines, using UBA1 rabbit monoclonal antibody (STJ11102462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Confocal imaging of paraffin-embedded Human colon cancer tissue using UBA1 Rabbit monoclonal antibody (STJ11102462, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded human esophageal using UBA1 rabbit monoclonal antibody (STJ11102462) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of UBA1 in paraffin-embedded human esophageal using UBA1 Rabbit monoclonal antibody (STJ11102462) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using UBA1 rabbit monoclonal antibody (STJ11102462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using UBA1 Rabbit monoclonal antibody (STJ11102462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-UBA1 antibody (959-1058) [S2MR] (STJ11102462)

SKU:
STJ11102462

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:100-1:1000
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBA1
Gene ID: 7317
Uniprot ID: UBA1_HUMAN
Immunogen Region: 959-1058
Specificity: A synthetic peptide corresponding to a sequence within amino acids 959-1058 of human UBA1 (P22314).
Immunogen Sequence: EVQGLQPNGEEMTLKQFLDY FKTEHKLEITMLSQGVSMLY SFFMPAAKLKERLDQPMTEI VSRVSKRKLGRHVRALVLEL CCNDESGEDVEVPYVRYTIR
Tissue Specificity Detected in erythrocytes (at protein level). Ubiquitous.
Post Translational Modifications ISGylated.
Function Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.
Protein Name Ubiquitin-Like Modifier-Activating Enzyme 1
Protein A1s9
Ubiquitin-Activating Enzyme E1
Database Links Reactome: R-HSA-8866652
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Mitochondrion
Nucleus
Isoform 1: Nucleus
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Ubiquitin-Like Modifier-Activating Enzyme 1 antibody
Anti-Protein A1s9 antibody
Anti-Ubiquitin-Activating Enzyme E1 antibody
Anti-UBA1 antibody
Anti-A1S9T antibody
Anti-UBE1 antibody

Information sourced from Uniprot.org

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