| Short Description : | Rabbit polyclonal U2AF1 (1-100) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:100-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | U2AF1 |
| Gene ID: | 7307 |
| Uniprot ID: | U2AF1_HUMAN |
| Immunogen Region: | 1-100 |
| Immunogen Sequence: | MAEYLASIFGTEKDKVNCSF YFKIGACRHGDRCSRLHNKP TFSQTIALLNIYRNPQNSSQ SADGLRCAVSDVEMQEHYDE FFEEVFTEMEEKYGEVEEMN |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1). |
| Function | Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. |
| Protein Name | Splicing Factor U2af 35 Kda SubunitU2 Auxiliary Factor 35 Kda SubunitU2 Small Nuclear Rna Auxiliary Factor 1U2 Snrnp Auxiliary Factor Small Subunit |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856 |
| Cellular Localisation | NucleusNucleus Speckle |
| Alternative Antibody Names | Anti-Splicing Factor U2af 35 Kda Subunit antibodyAnti-U2 Auxiliary Factor 35 Kda Subunit antibodyAnti-U2 Small Nuclear Rna Auxiliary Factor 1 antibodyAnti-U2 Snrnp Auxiliary Factor Small Subunit antibodyAnti-U2AF1 antibodyAnti-U2AF35 antibodyAnti-U2AFBP antibodyAnti-FP793 antibody |
Information sourced from Uniprot.org
